Journal: PLoS ONE

Article Title: The Proprotein Convertase Furin Contributes to Rhabdomyosarcoma Malignancy by Promoting Vascularization, Migration and Invasion

doi: 10.1371/journal.pone.0161396

Figure Lengend Snippet: A) mRNA levels of all nine proprotein convertases (PCs) were determined by qRT-PCR in 5 Ewing sarcoma, 8 osteosarcoma and 20 rhabdomyosarcoma (RMS) cell lines. Shown are levels relative to GAPDH expression. B) Protein levels of proform and mature furin were assessed by immunoblotting in 20 different RMS cell lines. C) Endogenous furin activity of selected RMS cell lines: Rh36, RD (eRMS), Rh3, Rh4, Rh30, Rh41 and RMS13 (aRMS). Furin activity deficient cells LoVo cells serve as negative control. Furin was captured from cell lysates on anti-furin antibody coated plates and furin activity was measured by addition of the fluorogenic substrate Boc-RVRR-AMC after 6h. Displayed are values normalized by background subtraction.

Article Snippet: Black FluoroNunc 96 well plates (MaxiSorp surface, Nunc, Thermo Scientific) were coated with goat anti human furin antibody (AF1503, R&D Systems) at 10 μg/ml in 50 mM Na 2 CO 3 , pH 9.6 (50 μl/ well) for 8h at RT, protected from light.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Activity Assay, Negative Control

Journal: Cell Reports

Article Title: TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions

doi: 10.1016/j.celrep.2019.08.050

Figure Lengend Snippet:

Article Snippet: Anti-human Furin (clone 222722) AF647 , R&D Systems , Cat# IC1503R-100UG; https://www.rndsystems.com/products/human-furin-alexa-fluor-647-conjugated-antibody-222722_ic1503r.

Techniques: Virus, Recombinant, Reverse Transcription, Activation Assay, Staining, Software